Ⅰ. Introduction
1. Background and purpose of the study
o Background
ㅇ Due to issues regarding animal rights, the economic burden of vertebrate testing, and the reliability of animal test results, the international community has shifted the paradigm of chemical safety assessment methods from animal testing to alternative test methods.
ㅇ Korea urgently needs to switch chemical safety assessment methodology of chemicals, biocides, and household chemical products to alternative test methods.
- The Ministry of Environment (MoE) estimates that more than 100,000 risk assessments need to be conducted by 2030, but currently less than 10% of the assessments utilize alternative methods in the process.

o Study goals
ㅇ This study aims to review the current status of technology development and policy utilization of animal alternative test methods in Korea, analyze laws, systems, and policy trends of alternative test methods in other countries, and propose legal and institutional improvement measures to promote animal alternative test methods for chemical safety assessment.

Ⅱ. Definition and Scope of Alternative Testing Methods
1. Definition of alternative test methods
ㅇ Animal alternative testing involves replacing traditionally conducted animal testing with non-destructive methods or alternatives.
ㅇ For the purposes of this study, we define ‘alternative test methods’ as technologies, methods, approaches, and harmonized utilization of these methods that do not involve the use of animals and can be utilized to determine the hazards and risks of chemicals.

2. Scope of alternative test m